Uncapped Dragons centre Aneurin Owen and scrum-half Che Hope have been included in Wales' squad for the Nations Championship.
Steve Tandy has selected a 39-strong group for the November Tests, with hooker Dewi Lake named as captain.
The head coach calls up Dragons lock Seb Davies and Cardiff number eight Taine Basham for the first time since he took charge of the national side.
Cardiff loose-head prop Rhys Barratt, who played in the summer fixture against the Barbarians, is the third uncapped player in the squad, while Dragons wing Rio Dyer earns a recall.
Leicester flanker Tommy Reffell and fit-again number eight Taulupe Faletau are among the notable absentees.
Wales face Japan, New Zealand and Australia in Cardiff and finish against a southern hemisphere opponent on finals weekend at Twickenham.
Owen was a member of the Wales squad in the summer of 2021 but did not feature in the Tests against Canada or Argentina.
The 26-year-old from Newport has been a key member of the Dragons side that has built on an encouraging 2025-26 with a promising start to the season.
Owen gets his chance in the absence of injured Cardiff centre Ben Thomas to join Eddie James, Joe Hawkins, Max Llewellyn and Louie Hennessey as a midfield option.
Hope, 23, is brought in for a first taste of international camp and joins Tomos Williams and Reuben Morgan-Williams, with Kieran Hardy facing a lengthy lay-off after a knee injury.
There is no place for in-form Scarlets wing Macs Page, but Dragons' Dyer, who has also started the season strongly, does earn a recall.
The 26-year-old won the most recent of his 24 caps against South Africa last November.
Leicester's Gabriel Hamer-Webb, who made his Wales debut against Scotland in the Six Nations, also returns after missing out in the summer.
Lock Davies, who moved from Cardiff to Dragons in the summer of 2025, will aim to win his first cap since the 2022 Six Nations.
Dynamic back-row forward Basham has started the season strongly for Cardiff and gets the nod ahead of fit-again Faletau, who made his first appearance of the season for Cardiff at Northampton on Sunday.
Wales' back-row riches mean that there is no place in the squad for specialist open-side Reffell, who featured in the summer Tests and has been in fine form for Leicester.
Lock Dafydd Jenkins, tight-heads Tomas Francis and Archie Griffin, hooker Liam Belcher and centre Hennessey return after being unavailable for the July Tests against Fiji, Argentina and South Africa.
Belcher gets the nod ahead of Elliot Dee, the leading try scorer in the United Rugby Championship (URC), as the third hooker behind captain Lake and Ryan Elias.
Tandy said: "It's great to welcome back the players who were unavailable for the first three rounds of the Nations Championship.
"Added to that, there are a number of players who've been in great form for their clubs and so we wanted to give them an opportunity in the squad this autumn."
The squad features 14 players from clubs in England plus Montpellier lock Adam Beard.
There are seven call-ups for Cardiff and Scarlets, six for Dragons and four from Ospreys.
There is a round of URC action this weekend and back-row forward Taine Plumtree and centre Hawkins have been allowed to travel to South Africa with Scarlets for their fixture against Stormers on Saturday.
Forwards: Rhys Barratt, Rhys Carre, Nicky Smith, Gareth Thomas, Dewi Lake (capt), Liam Belcher, Ryan Elias, Tomas Francis, Archie Griffin, Dillon Lewis, Adam Beard, Ben Carter, Seb Davies, Dafydd Jenkins, Teddy Williams, Taine Basham, James Botham, Kane James, Alex Mann, Jac Morgan, Taine Plumtree, Aaron Wainwright.
Backs: Che Hope, Reuben Morgan-Williams, Tomos Williams, Sam Costelow, Dan Edwards, Joe Hawkins, Louie Hennessey, Eddie James, Max Llewellyn, Aneurin Owen, Josh Adams, Rio Dyer, Gabriel Hamer-Webb, Ellis Mee, Blair Murray, Louis Rees-Zammit, Tom Rogers.