The rock group will provide halftime entertainment on November 25, when the professional American football league, the NFL, plays a game for the first time on the evening before Thanksgiving.
The game between the LA Rams and the Green Bay Packers will be broadcast on Netflix and is one of the five NFL games the service shows in Sweden.
As they celebrate their 50th anniversary, U2, who made history in 2002 with their acclaimed Super Bowl performance, will headline the halftime show.
"They don't just perform - they create iconic moments that audiences will remember forever. It will be an unforgettable evening," Netflix CEO Ted Sarandos said in a statement.
Last year, Netflix's Christmas Day broadcast was the most-streamed regular-season game in NFL history, with Snoop Dogg providing halftime entertainment.